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rabbit anti phosphorylated p akt  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc rabbit anti phosphorylated p akt
    Rabbit Anti Phosphorylated P Akt, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/phosphorylated+akt/pm41763584-177-36-77
    Average 86 stars, based on 1 article reviews
    rabbit anti phosphorylated p akt - by Bioz Stars, 2026-09
    86/100 stars

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    Related Articles

    Expressing:

    Article Title: MyD88 Inhibition Ameliorates Diabetes-Induced Hepatic Inflammation and Gluconeogenesis Through Adipose IL-10 Induction
    Article Snippet: Amplification was performed using a StepOnePlusTM Real-Time PCR System (Applied Biosystems, Foster City, CA, USA). .. Protein expression of phosphorylated Akt (pAkt; Cell Signaling, #4060), Akt (#4691), JNK (#9252), pJNK (#9251), ERK (#4695), and pERK (#9101) was determined by Western blotting. .. Tissue samples were homogenized in protein extraction buffer (Sigma-Aldrich, St. Louis, MO, USA) supplemented with a protease inhibitor cocktail (Roche, Basel, Switzerland).

    Article Title: MyD88 Inhibition Ameliorates Diabetes-Induced Hepatic Inflammation and Gluconeogenesis Through Adipose IL-10 Induction.
    Article Snippet: Amplification was performed using a StepOnePlusTM Real-Time PCR System (Applied Biosystems, Foster City, CA, USA). .. Protein expression of phosphorylated Akt (pAkt; Cell Signaling, #4060), Akt (#4691), JNK (#9252), pJNK (#9251), ERK (#4695), and pERK (#9101) was determined by Western blotting. .. Tissue samples were homogenized in protein extraction buffer (Sigma-Aldrich, St. Louis, MO, USA) supplemented with a protease inhibitor cocktail (Roche, Basel, Switzerland).

    Western Blot:

    Article Title: MyD88 Inhibition Ameliorates Diabetes-Induced Hepatic Inflammation and Gluconeogenesis Through Adipose IL-10 Induction
    Article Snippet: Amplification was performed using a StepOnePlusTM Real-Time PCR System (Applied Biosystems, Foster City, CA, USA). .. Protein expression of phosphorylated Akt (pAkt; Cell Signaling, #4060), Akt (#4691), JNK (#9252), pJNK (#9251), ERK (#4695), and pERK (#9101) was determined by Western blotting. .. Tissue samples were homogenized in protein extraction buffer (Sigma-Aldrich, St. Louis, MO, USA) supplemented with a protease inhibitor cocktail (Roche, Basel, Switzerland).

    Article Title: MyD88 Inhibition Ameliorates Diabetes-Induced Hepatic Inflammation and Gluconeogenesis Through Adipose IL-10 Induction.
    Article Snippet: Amplification was performed using a StepOnePlusTM Real-Time PCR System (Applied Biosystems, Foster City, CA, USA). .. Protein expression of phosphorylated Akt (pAkt; Cell Signaling, #4060), Akt (#4691), JNK (#9252), pJNK (#9251), ERK (#4695), and pERK (#9101) was determined by Western blotting. .. Tissue samples were homogenized in protein extraction buffer (Sigma-Aldrich, St. Louis, MO, USA) supplemented with a protease inhibitor cocktail (Roche, Basel, Switzerland).

    Incubation:

    Article Title: A Novel Role for the Small Molecule Cinnamaldehyde in Protecting Against P. gingivalis -Induced Endothelial Dysfunction in Mice: Involvement of PPARγ/Akt/eNOS and Nrf2/ARE Signaling.
    Article Snippet: .. Following transfer, membranes were incubated in blocking solution containing 5% nonfat dry milk for 1 h at room temperature and then probed with primary antibodies recognizing PPARγ (sc-7273), GSK3β (sc-377213), Keap1 (sc-515432), and NQO1 (sc-376023, Santa Cruz Biotechnology, Dallas, TX, USA), AKT (9272) and phosphorylated AKT (9271, Cell Signaling Technology, Danvers, MA, USA), or endothelial nitric oxide synthase (eNOS; ab19956; Abcam, Cambridge, MA, USA). ..

    Article Title: A Novel Role for the Small Molecule Cinnamaldehyde in Protecting Against P. gingivalis –Induced Endothelial Dysfunction in Mice: Involvement of PPARγ/Akt/eNOS and Nrf2/ARE Signaling
    Article Snippet: .. Following transfer, membranes were incubated in blocking solution containing 5% nonfat dry milk for 1 h at room temperature and then probed with primary antibodies recognizing PPARγ (sc-7273), GSK3β (sc-377213), Keap1 (sc-515432), and NQO1 (sc-376023, Santa Cruz Biotechnology, Dallas, TX, USA), AKT (9272) and phosphorylated AKT (9271, Cell Signaling Technology, Danvers, MA, USA), or endothelial nitric oxide synthase (eNOS; ab19956; Abcam, Cambridge, MA, USA). ..

    Blocking Assay:

    Article Title: A Novel Role for the Small Molecule Cinnamaldehyde in Protecting Against P. gingivalis -Induced Endothelial Dysfunction in Mice: Involvement of PPARγ/Akt/eNOS and Nrf2/ARE Signaling.
    Article Snippet: .. Following transfer, membranes were incubated in blocking solution containing 5% nonfat dry milk for 1 h at room temperature and then probed with primary antibodies recognizing PPARγ (sc-7273), GSK3β (sc-377213), Keap1 (sc-515432), and NQO1 (sc-376023, Santa Cruz Biotechnology, Dallas, TX, USA), AKT (9272) and phosphorylated AKT (9271, Cell Signaling Technology, Danvers, MA, USA), or endothelial nitric oxide synthase (eNOS; ab19956; Abcam, Cambridge, MA, USA). ..

    Article Title: A Novel Role for the Small Molecule Cinnamaldehyde in Protecting Against P. gingivalis –Induced Endothelial Dysfunction in Mice: Involvement of PPARγ/Akt/eNOS and Nrf2/ARE Signaling
    Article Snippet: .. Following transfer, membranes were incubated in blocking solution containing 5% nonfat dry milk for 1 h at room temperature and then probed with primary antibodies recognizing PPARγ (sc-7273), GSK3β (sc-377213), Keap1 (sc-515432), and NQO1 (sc-376023, Santa Cruz Biotechnology, Dallas, TX, USA), AKT (9272) and phosphorylated AKT (9271, Cell Signaling Technology, Danvers, MA, USA), or endothelial nitric oxide synthase (eNOS; ab19956; Abcam, Cambridge, MA, USA). ..

    Polyacrylamide Gel Electrophoresis:

    Article Title: Decreased Kinase Activity of the VEGFR3 Variant c.3175G>C Associated with Primary Lymphedema.
    Article Snippet: .. Samples underwent separation via 10% PAGE under denaturing conditions before being transferred to a PVDF membrane and probed with antibodies specific to phosphorylated VEGFR3 (Tyr1230, #AF3676, Affinity Biosciences, Liyang, China), phosphorylated AKT (Ser273, #4060, Cell Signaling Technologies, Danvers, MA, USA), total VEGFR3 (#AF4201, Affinity Biosciences, China), total AKT (#AF6259, Affinity Biosciences, China), and GAPDH (#CAB932Hu01, Cloud-clone, Wuhan, China) as a loading control. ..

    Membrane:

    Article Title: Decreased Kinase Activity of the VEGFR3 Variant c.3175G>C Associated with Primary Lymphedema.
    Article Snippet: .. Samples underwent separation via 10% PAGE under denaturing conditions before being transferred to a PVDF membrane and probed with antibodies specific to phosphorylated VEGFR3 (Tyr1230, #AF3676, Affinity Biosciences, Liyang, China), phosphorylated AKT (Ser273, #4060, Cell Signaling Technologies, Danvers, MA, USA), total VEGFR3 (#AF4201, Affinity Biosciences, China), total AKT (#AF6259, Affinity Biosciences, China), and GAPDH (#CAB932Hu01, Cloud-clone, Wuhan, China) as a loading control. ..

    Control:

    Article Title: Decreased Kinase Activity of the VEGFR3 Variant c.3175G>C Associated with Primary Lymphedema.
    Article Snippet: .. Samples underwent separation via 10% PAGE under denaturing conditions before being transferred to a PVDF membrane and probed with antibodies specific to phosphorylated VEGFR3 (Tyr1230, #AF3676, Affinity Biosciences, Liyang, China), phosphorylated AKT (Ser273, #4060, Cell Signaling Technologies, Danvers, MA, USA), total VEGFR3 (#AF4201, Affinity Biosciences, China), total AKT (#AF6259, Affinity Biosciences, China), and GAPDH (#CAB932Hu01, Cloud-clone, Wuhan, China) as a loading control. ..

    Article Title: Extracellular Vesicles from Bone Marrow Mesenchymal Stem Cells Modulate Proliferation, Migration, and Chemosensitivity in Ovarian Cancer Cells
    Article Snippet: .. Tumor lysates were analyzed for phosphorylated AKT (Ser473; p-AKT) and total AKT, the anti-apoptotic protein BCL-2, the angiogenesis marker CD31, vimentin, and VEGFA. β-Actin served as the loading control (all from Cell Signaling, Danvers, MA, USA). ..

    Article Title: Extracellular Vesicles from Bone Marrow Mesenchymal Stem Cells Modulate Proliferation, Migration, and Chemosensitivity in Ovarian Cancer Cells.
    Article Snippet: .. Tumor lysates were analyzed for phosphorylated AKT (Ser473; p-AKT) and total AKT, the anti-apoptotic protein BCL-2, the angiogenesis marker CD31, vimentin, and VEGFA. β-Actin served as the loading control (all from Cell Signaling, Danvers, MA, USA). ..

    Marker:

    Article Title: Extracellular Vesicles from Bone Marrow Mesenchymal Stem Cells Modulate Proliferation, Migration, and Chemosensitivity in Ovarian Cancer Cells
    Article Snippet: .. Tumor lysates were analyzed for phosphorylated AKT (Ser473; p-AKT) and total AKT, the anti-apoptotic protein BCL-2, the angiogenesis marker CD31, vimentin, and VEGFA. β-Actin served as the loading control (all from Cell Signaling, Danvers, MA, USA). ..

    Article Title: Extracellular Vesicles from Bone Marrow Mesenchymal Stem Cells Modulate Proliferation, Migration, and Chemosensitivity in Ovarian Cancer Cells.
    Article Snippet: .. Tumor lysates were analyzed for phosphorylated AKT (Ser473; p-AKT) and total AKT, the anti-apoptotic protein BCL-2, the angiogenesis marker CD31, vimentin, and VEGFA. β-Actin served as the loading control (all from Cell Signaling, Danvers, MA, USA). ..



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    Image Search Results


    TRIM46 depletion inhibits cell proliferation by regulating the Akt signaling pathway. (A) Cell proliferation of A549/DDP cells transduced with TRIM46-knockdown lentiviruses. (B) Western blot analysis of p-Akt, Akt, caspase 3, cleaved-caspase 3 and RAD51 expression in A549/DDP cells transduced with TRIM46-knockdown lentiviruses. (C) Cell proliferation of A549 cells transduced with TRIM46-overexpression lentivirus and/or treated with LY294002 (20 µM) or vehicle. (D) Western blot analysis of p-Akt, Akt, caspase 3, cleaved-caspase 3 and RAD51 in A549 cells transduced with TRIM46-overexpression lentivirus and/or treated with LY294002 (20 µM) or vehicle. ***P<0.001 vs. shNC or Vector + Vehicle; ### P<0.001 vs. TRIM46 + Vehicle. TRIM46, tripartite motif 46; DDP, cisplatin; sh, short hairpin; NC, negative control; OD, optical density; p-, phosphorylated.

    Journal: Oncology Reports

    Article Title: TRIM46 deficiency-induced DNA damage enhances the sensitivity of cisplatin in non-small cell lung cancer by regulating the Akt signaling pathway

    doi: 10.3892/or.2026.9063

    Figure Lengend Snippet: TRIM46 depletion inhibits cell proliferation by regulating the Akt signaling pathway. (A) Cell proliferation of A549/DDP cells transduced with TRIM46-knockdown lentiviruses. (B) Western blot analysis of p-Akt, Akt, caspase 3, cleaved-caspase 3 and RAD51 expression in A549/DDP cells transduced with TRIM46-knockdown lentiviruses. (C) Cell proliferation of A549 cells transduced with TRIM46-overexpression lentivirus and/or treated with LY294002 (20 µM) or vehicle. (D) Western blot analysis of p-Akt, Akt, caspase 3, cleaved-caspase 3 and RAD51 in A549 cells transduced with TRIM46-overexpression lentivirus and/or treated with LY294002 (20 µM) or vehicle. ***P<0.001 vs. shNC or Vector + Vehicle; ### P<0.001 vs. TRIM46 + Vehicle. TRIM46, tripartite motif 46; DDP, cisplatin; sh, short hairpin; NC, negative control; OD, optical density; p-, phosphorylated.

    Article Snippet: The anti-Akt (cat. no. 9272; 1:1,000 for western blotting), anti-phosphorylated (p-)Akt (cat. no. 4060; 1:1,000 for western blotting) and anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibodies (cat. no. 5174; 1:3,000 for western blotting) were purchased from Cell Signaling Technology, Inc. 3,3′-Diaminobenzidine (DAB) detection kit (cat. no. 34002), Roswell Park Memorial Institute 1640 medium (RPMI-1640; cat. no. 11875101) and fetal bovine serum (FBS; cat. no. 10099141C) were purchased from Gibco (Thermo Fisher Scientific, Inc.).

    Techniques: Transduction, Knockdown, Western Blot, Expressing, Over Expression, Plasmid Preparation, Negative Control

    Mechanism of TRIM46 deficiency-induced DNA damage, enhancing the sensitivity of DDP in NSCLC by regulating Akt signaling pathway. (A) TRIM46 expression was positively associated with DDP resistance in NSCLC tissues. (B) TRIM46 overexpression significantly suppressed DDP-induced apoptosis and enhanced DDP resistance in A549 cells. (C) TRIM46 knockdown induced DNA damage by modulating the protein levels of p-AKT, RAD51, caspase 3, and cleaved-caspase 3, thereby resulting in cell proliferation inhibition in NSCLC cells. (D) TRIM46 knockdown increased the sensitivity of xenograft tumors to DDP treatment. NSCLC, non-small cell lung cancer; TRIM46, tripartite motif 46; DDP, cisplatin; p-, phosphorylated.

    Journal: Oncology Reports

    Article Title: TRIM46 deficiency-induced DNA damage enhances the sensitivity of cisplatin in non-small cell lung cancer by regulating the Akt signaling pathway

    doi: 10.3892/or.2026.9063

    Figure Lengend Snippet: Mechanism of TRIM46 deficiency-induced DNA damage, enhancing the sensitivity of DDP in NSCLC by regulating Akt signaling pathway. (A) TRIM46 expression was positively associated with DDP resistance in NSCLC tissues. (B) TRIM46 overexpression significantly suppressed DDP-induced apoptosis and enhanced DDP resistance in A549 cells. (C) TRIM46 knockdown induced DNA damage by modulating the protein levels of p-AKT, RAD51, caspase 3, and cleaved-caspase 3, thereby resulting in cell proliferation inhibition in NSCLC cells. (D) TRIM46 knockdown increased the sensitivity of xenograft tumors to DDP treatment. NSCLC, non-small cell lung cancer; TRIM46, tripartite motif 46; DDP, cisplatin; p-, phosphorylated.

    Article Snippet: The anti-Akt (cat. no. 9272; 1:1,000 for western blotting), anti-phosphorylated (p-)Akt (cat. no. 4060; 1:1,000 for western blotting) and anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibodies (cat. no. 5174; 1:3,000 for western blotting) were purchased from Cell Signaling Technology, Inc. 3,3′-Diaminobenzidine (DAB) detection kit (cat. no. 34002), Roswell Park Memorial Institute 1640 medium (RPMI-1640; cat. no. 11875101) and fetal bovine serum (FBS; cat. no. 10099141C) were purchased from Gibco (Thermo Fisher Scientific, Inc.).

    Techniques: Expressing, Over Expression, Knockdown, Inhibition