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akt phosphorylation inhibitor ly294002  (MedChemExpress)
96
MedChemExpress akt phosphorylation inhibitor ly294002
<t>LY294002</t> suppresses exosomes derived from CO 2 laser-preconditioned adipose-derived mesenchymal stem cells -induced angiogenesis. b P < 0.01; c P < 0.001. Data are presented as mean ± SD ( n = 3). A and B: Western blot analysis of phosphorylated protein kinase B, protein kinase B, hypoxia-inducible factor-1α, and vascular endothelial growth factor-A protein expression levels in human umbilical vein endothelial cells (HUVECs) treated with LY294002 vs negative control and LY294002 + exosomes derived from CO 2 laser-preconditioned adipose-derived mesenchymal stem cells vs exosomes derived from CO 2 laser-preconditioned adipose-derived mesenchymal stem cells; C and D: 5-Ethynyl-2’-deoxyuridine incorporation assay to assess HUVEC proliferation. Scale bar = 100 μm; E: Cell Counting Kit-8 assay to evaluate HUVEC viability; F and G: Transwell migration assay to assess the migratory capacity of HUVECs. Scale bar = 200 μm; H and I: In vitro wound healing assay to evaluate cell migration. Scale bar = 200 μm; J and K: Tube formation assay to examine the formation of capillary-like structures by HUVECs. Scale bar = 200 μm. EdU: 5-Ethynyl-2’-deoxyuridine; NC: Negative control; CO 2 laser-Exos: Exosomes derived from CO 2 laser-preconditioned adipose-derived mesenchymal stem cells; AKT: Protein kinase B; p-AKT: Phosphorylated protein kinase B; HIF-1α: Hypoxia-inducible factor-1α; VEGF-A: Vascular endothelial growth factor-A.
Akt Phosphorylation Inhibitor Ly294002, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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phosphorylated akt protein  (Proteintech)
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Proteintech phosphorylated akt protein
<t>LY294002</t> suppresses exosomes derived from CO 2 laser-preconditioned adipose-derived mesenchymal stem cells -induced angiogenesis. b P < 0.01; c P < 0.001. Data are presented as mean ± SD ( n = 3). A and B: Western blot analysis of phosphorylated protein kinase B, protein kinase B, hypoxia-inducible factor-1α, and vascular endothelial growth factor-A protein expression levels in human umbilical vein endothelial cells (HUVECs) treated with LY294002 vs negative control and LY294002 + exosomes derived from CO 2 laser-preconditioned adipose-derived mesenchymal stem cells vs exosomes derived from CO 2 laser-preconditioned adipose-derived mesenchymal stem cells; C and D: 5-Ethynyl-2’-deoxyuridine incorporation assay to assess HUVEC proliferation. Scale bar = 100 μm; E: Cell Counting Kit-8 assay to evaluate HUVEC viability; F and G: Transwell migration assay to assess the migratory capacity of HUVECs. Scale bar = 200 μm; H and I: In vitro wound healing assay to evaluate cell migration. Scale bar = 200 μm; J and K: Tube formation assay to examine the formation of capillary-like structures by HUVECs. Scale bar = 200 μm. EdU: 5-Ethynyl-2’-deoxyuridine; NC: Negative control; CO 2 laser-Exos: Exosomes derived from CO 2 laser-preconditioned adipose-derived mesenchymal stem cells; AKT: Protein kinase B; p-AKT: Phosphorylated protein kinase B; HIF-1α: Hypoxia-inducible factor-1α; VEGF-A: Vascular endothelial growth factor-A.
Phosphorylated Akt Protein, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/phosphorylated akt protein/product/Proteintech
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phosphorylated akt protein - by Bioz Stars, 2026-02
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anti phosphorylated akt  (Proteintech)
96
Proteintech anti phosphorylated akt
<t>LY294002</t> suppresses exosomes derived from CO 2 laser-preconditioned adipose-derived mesenchymal stem cells -induced angiogenesis. b P < 0.01; c P < 0.001. Data are presented as mean ± SD ( n = 3). A and B: Western blot analysis of phosphorylated protein kinase B, protein kinase B, hypoxia-inducible factor-1α, and vascular endothelial growth factor-A protein expression levels in human umbilical vein endothelial cells (HUVECs) treated with LY294002 vs negative control and LY294002 + exosomes derived from CO 2 laser-preconditioned adipose-derived mesenchymal stem cells vs exosomes derived from CO 2 laser-preconditioned adipose-derived mesenchymal stem cells; C and D: 5-Ethynyl-2’-deoxyuridine incorporation assay to assess HUVEC proliferation. Scale bar = 100 μm; E: Cell Counting Kit-8 assay to evaluate HUVEC viability; F and G: Transwell migration assay to assess the migratory capacity of HUVECs. Scale bar = 200 μm; H and I: In vitro wound healing assay to evaluate cell migration. Scale bar = 200 μm; J and K: Tube formation assay to examine the formation of capillary-like structures by HUVECs. Scale bar = 200 μm. EdU: 5-Ethynyl-2’-deoxyuridine; NC: Negative control; CO 2 laser-Exos: Exosomes derived from CO 2 laser-preconditioned adipose-derived mesenchymal stem cells; AKT: Protein kinase B; p-AKT: Phosphorylated protein kinase B; HIF-1α: Hypoxia-inducible factor-1α; VEGF-A: Vascular endothelial growth factor-A.
Anti Phosphorylated Akt, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti phosphorylated akt/product/Proteintech
Average 96 stars, based on 1 article reviews
anti phosphorylated akt - by Bioz Stars, 2026-02
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mouse anti phosphorylated akt  (Proteintech)
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Proteintech mouse anti phosphorylated akt
<t>LY294002</t> suppresses exosomes derived from CO 2 laser-preconditioned adipose-derived mesenchymal stem cells -induced angiogenesis. b P < 0.01; c P < 0.001. Data are presented as mean ± SD ( n = 3). A and B: Western blot analysis of phosphorylated protein kinase B, protein kinase B, hypoxia-inducible factor-1α, and vascular endothelial growth factor-A protein expression levels in human umbilical vein endothelial cells (HUVECs) treated with LY294002 vs negative control and LY294002 + exosomes derived from CO 2 laser-preconditioned adipose-derived mesenchymal stem cells vs exosomes derived from CO 2 laser-preconditioned adipose-derived mesenchymal stem cells; C and D: 5-Ethynyl-2’-deoxyuridine incorporation assay to assess HUVEC proliferation. Scale bar = 100 μm; E: Cell Counting Kit-8 assay to evaluate HUVEC viability; F and G: Transwell migration assay to assess the migratory capacity of HUVECs. Scale bar = 200 μm; H and I: In vitro wound healing assay to evaluate cell migration. Scale bar = 200 μm; J and K: Tube formation assay to examine the formation of capillary-like structures by HUVECs. Scale bar = 200 μm. EdU: 5-Ethynyl-2’-deoxyuridine; NC: Negative control; CO 2 laser-Exos: Exosomes derived from CO 2 laser-preconditioned adipose-derived mesenchymal stem cells; AKT: Protein kinase B; p-AKT: Phosphorylated protein kinase B; HIF-1α: Hypoxia-inducible factor-1α; VEGF-A: Vascular endothelial growth factor-A.
Mouse Anti Phosphorylated Akt, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse anti phosphorylated akt/product/Proteintech
Average 96 stars, based on 1 article reviews
mouse anti phosphorylated akt - by Bioz Stars, 2026-02
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phosphorylated p protein kinase b  (Proteintech)
96
Proteintech phosphorylated p protein kinase b
<t>LY294002</t> suppresses exosomes derived from CO 2 laser-preconditioned adipose-derived mesenchymal stem cells -induced angiogenesis. b P < 0.01; c P < 0.001. Data are presented as mean ± SD ( n = 3). A and B: Western blot analysis of phosphorylated protein kinase B, protein kinase B, hypoxia-inducible factor-1α, and vascular endothelial growth factor-A protein expression levels in human umbilical vein endothelial cells (HUVECs) treated with LY294002 vs negative control and LY294002 + exosomes derived from CO 2 laser-preconditioned adipose-derived mesenchymal stem cells vs exosomes derived from CO 2 laser-preconditioned adipose-derived mesenchymal stem cells; C and D: 5-Ethynyl-2’-deoxyuridine incorporation assay to assess HUVEC proliferation. Scale bar = 100 μm; E: Cell Counting Kit-8 assay to evaluate HUVEC viability; F and G: Transwell migration assay to assess the migratory capacity of HUVECs. Scale bar = 200 μm; H and I: In vitro wound healing assay to evaluate cell migration. Scale bar = 200 μm; J and K: Tube formation assay to examine the formation of capillary-like structures by HUVECs. Scale bar = 200 μm. EdU: 5-Ethynyl-2’-deoxyuridine; NC: Negative control; CO 2 laser-Exos: Exosomes derived from CO 2 laser-preconditioned adipose-derived mesenchymal stem cells; AKT: Protein kinase B; p-AKT: Phosphorylated protein kinase B; HIF-1α: Hypoxia-inducible factor-1α; VEGF-A: Vascular endothelial growth factor-A.
Phosphorylated P Protein Kinase B, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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phosphorylated p protein kinase b - by Bioz Stars, 2026-02
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phosphorylated akt monoclonal mouse anti human ser473 antibody  (Proteintech)
96
Proteintech phosphorylated akt monoclonal mouse anti human ser473 antibody
<t>LY294002</t> suppresses exosomes derived from CO 2 laser-preconditioned adipose-derived mesenchymal stem cells -induced angiogenesis. b P < 0.01; c P < 0.001. Data are presented as mean ± SD ( n = 3). A and B: Western blot analysis of phosphorylated protein kinase B, protein kinase B, hypoxia-inducible factor-1α, and vascular endothelial growth factor-A protein expression levels in human umbilical vein endothelial cells (HUVECs) treated with LY294002 vs negative control and LY294002 + exosomes derived from CO 2 laser-preconditioned adipose-derived mesenchymal stem cells vs exosomes derived from CO 2 laser-preconditioned adipose-derived mesenchymal stem cells; C and D: 5-Ethynyl-2’-deoxyuridine incorporation assay to assess HUVEC proliferation. Scale bar = 100 μm; E: Cell Counting Kit-8 assay to evaluate HUVEC viability; F and G: Transwell migration assay to assess the migratory capacity of HUVECs. Scale bar = 200 μm; H and I: In vitro wound healing assay to evaluate cell migration. Scale bar = 200 μm; J and K: Tube formation assay to examine the formation of capillary-like structures by HUVECs. Scale bar = 200 μm. EdU: 5-Ethynyl-2’-deoxyuridine; NC: Negative control; CO 2 laser-Exos: Exosomes derived from CO 2 laser-preconditioned adipose-derived mesenchymal stem cells; AKT: Protein kinase B; p-AKT: Phosphorylated protein kinase B; HIF-1α: Hypoxia-inducible factor-1α; VEGF-A: Vascular endothelial growth factor-A.
Phosphorylated Akt Monoclonal Mouse Anti Human Ser473 Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/phosphorylated akt monoclonal mouse anti human ser473 antibody/product/Proteintech
Average 96 stars, based on 1 article reviews
phosphorylated akt monoclonal mouse anti human ser473 antibody - by Bioz Stars, 2026-02
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anti phosphorylated akt s473  (Proteintech)
96
Proteintech anti phosphorylated akt s473
<t>LY294002</t> suppresses exosomes derived from CO 2 laser-preconditioned adipose-derived mesenchymal stem cells -induced angiogenesis. b P < 0.01; c P < 0.001. Data are presented as mean ± SD ( n = 3). A and B: Western blot analysis of phosphorylated protein kinase B, protein kinase B, hypoxia-inducible factor-1α, and vascular endothelial growth factor-A protein expression levels in human umbilical vein endothelial cells (HUVECs) treated with LY294002 vs negative control and LY294002 + exosomes derived from CO 2 laser-preconditioned adipose-derived mesenchymal stem cells vs exosomes derived from CO 2 laser-preconditioned adipose-derived mesenchymal stem cells; C and D: 5-Ethynyl-2’-deoxyuridine incorporation assay to assess HUVEC proliferation. Scale bar = 100 μm; E: Cell Counting Kit-8 assay to evaluate HUVEC viability; F and G: Transwell migration assay to assess the migratory capacity of HUVECs. Scale bar = 200 μm; H and I: In vitro wound healing assay to evaluate cell migration. Scale bar = 200 μm; J and K: Tube formation assay to examine the formation of capillary-like structures by HUVECs. Scale bar = 200 μm. EdU: 5-Ethynyl-2’-deoxyuridine; NC: Negative control; CO 2 laser-Exos: Exosomes derived from CO 2 laser-preconditioned adipose-derived mesenchymal stem cells; AKT: Protein kinase B; p-AKT: Phosphorylated protein kinase B; HIF-1α: Hypoxia-inducible factor-1α; VEGF-A: Vascular endothelial growth factor-A.
Anti Phosphorylated Akt S473, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti phosphorylated akt s473 - by Bioz Stars, 2026-02
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anti phosphorylated akt antibody  (Proteintech)
96
Proteintech anti phosphorylated akt antibody
<t>LY294002</t> suppresses exosomes derived from CO 2 laser-preconditioned adipose-derived mesenchymal stem cells -induced angiogenesis. b P < 0.01; c P < 0.001. Data are presented as mean ± SD ( n = 3). A and B: Western blot analysis of phosphorylated protein kinase B, protein kinase B, hypoxia-inducible factor-1α, and vascular endothelial growth factor-A protein expression levels in human umbilical vein endothelial cells (HUVECs) treated with LY294002 vs negative control and LY294002 + exosomes derived from CO 2 laser-preconditioned adipose-derived mesenchymal stem cells vs exosomes derived from CO 2 laser-preconditioned adipose-derived mesenchymal stem cells; C and D: 5-Ethynyl-2’-deoxyuridine incorporation assay to assess HUVEC proliferation. Scale bar = 100 μm; E: Cell Counting Kit-8 assay to evaluate HUVEC viability; F and G: Transwell migration assay to assess the migratory capacity of HUVECs. Scale bar = 200 μm; H and I: In vitro wound healing assay to evaluate cell migration. Scale bar = 200 μm; J and K: Tube formation assay to examine the formation of capillary-like structures by HUVECs. Scale bar = 200 μm. EdU: 5-Ethynyl-2’-deoxyuridine; NC: Negative control; CO 2 laser-Exos: Exosomes derived from CO 2 laser-preconditioned adipose-derived mesenchymal stem cells; AKT: Protein kinase B; p-AKT: Phosphorylated protein kinase B; HIF-1α: Hypoxia-inducible factor-1α; VEGF-A: Vascular endothelial growth factor-A.
Anti Phosphorylated Akt Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti phosphorylated akt antibody/product/Proteintech
Average 96 stars, based on 1 article reviews
anti phosphorylated akt antibody - by Bioz Stars, 2026-02
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phosphorylated akt1  (Proteintech)
96
Proteintech phosphorylated akt1
<t>LY294002</t> suppresses exosomes derived from CO 2 laser-preconditioned adipose-derived mesenchymal stem cells -induced angiogenesis. b P < 0.01; c P < 0.001. Data are presented as mean ± SD ( n = 3). A and B: Western blot analysis of phosphorylated protein kinase B, protein kinase B, hypoxia-inducible factor-1α, and vascular endothelial growth factor-A protein expression levels in human umbilical vein endothelial cells (HUVECs) treated with LY294002 vs negative control and LY294002 + exosomes derived from CO 2 laser-preconditioned adipose-derived mesenchymal stem cells vs exosomes derived from CO 2 laser-preconditioned adipose-derived mesenchymal stem cells; C and D: 5-Ethynyl-2’-deoxyuridine incorporation assay to assess HUVEC proliferation. Scale bar = 100 μm; E: Cell Counting Kit-8 assay to evaluate HUVEC viability; F and G: Transwell migration assay to assess the migratory capacity of HUVECs. Scale bar = 200 μm; H and I: In vitro wound healing assay to evaluate cell migration. Scale bar = 200 μm; J and K: Tube formation assay to examine the formation of capillary-like structures by HUVECs. Scale bar = 200 μm. EdU: 5-Ethynyl-2’-deoxyuridine; NC: Negative control; CO 2 laser-Exos: Exosomes derived from CO 2 laser-preconditioned adipose-derived mesenchymal stem cells; AKT: Protein kinase B; p-AKT: Phosphorylated protein kinase B; HIF-1α: Hypoxia-inducible factor-1α; VEGF-A: Vascular endothelial growth factor-A.
Phosphorylated Akt1, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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LY294002 suppresses exosomes derived from CO 2 laser-preconditioned adipose-derived mesenchymal stem cells -induced angiogenesis. b P < 0.01; c P < 0.001. Data are presented as mean ± SD ( n = 3). A and B: Western blot analysis of phosphorylated protein kinase B, protein kinase B, hypoxia-inducible factor-1α, and vascular endothelial growth factor-A protein expression levels in human umbilical vein endothelial cells (HUVECs) treated with LY294002 vs negative control and LY294002 + exosomes derived from CO 2 laser-preconditioned adipose-derived mesenchymal stem cells vs exosomes derived from CO 2 laser-preconditioned adipose-derived mesenchymal stem cells; C and D: 5-Ethynyl-2’-deoxyuridine incorporation assay to assess HUVEC proliferation. Scale bar = 100 μm; E: Cell Counting Kit-8 assay to evaluate HUVEC viability; F and G: Transwell migration assay to assess the migratory capacity of HUVECs. Scale bar = 200 μm; H and I: In vitro wound healing assay to evaluate cell migration. Scale bar = 200 μm; J and K: Tube formation assay to examine the formation of capillary-like structures by HUVECs. Scale bar = 200 μm. EdU: 5-Ethynyl-2’-deoxyuridine; NC: Negative control; CO 2 laser-Exos: Exosomes derived from CO 2 laser-preconditioned adipose-derived mesenchymal stem cells; AKT: Protein kinase B; p-AKT: Phosphorylated protein kinase B; HIF-1α: Hypoxia-inducible factor-1α; VEGF-A: Vascular endothelial growth factor-A.

Journal: World Journal of Diabetes

Article Title: Fractional carbon dioxide laser-induced photothermal activation of mesenchymal stem cell-derived exosomes accelerates diabetic wound healing by enhancing angiogenesis

doi: 10.4239/wjd.v17.i1.112942

Figure Lengend Snippet: LY294002 suppresses exosomes derived from CO 2 laser-preconditioned adipose-derived mesenchymal stem cells -induced angiogenesis. b P < 0.01; c P < 0.001. Data are presented as mean ± SD ( n = 3). A and B: Western blot analysis of phosphorylated protein kinase B, protein kinase B, hypoxia-inducible factor-1α, and vascular endothelial growth factor-A protein expression levels in human umbilical vein endothelial cells (HUVECs) treated with LY294002 vs negative control and LY294002 + exosomes derived from CO 2 laser-preconditioned adipose-derived mesenchymal stem cells vs exosomes derived from CO 2 laser-preconditioned adipose-derived mesenchymal stem cells; C and D: 5-Ethynyl-2’-deoxyuridine incorporation assay to assess HUVEC proliferation. Scale bar = 100 μm; E: Cell Counting Kit-8 assay to evaluate HUVEC viability; F and G: Transwell migration assay to assess the migratory capacity of HUVECs. Scale bar = 200 μm; H and I: In vitro wound healing assay to evaluate cell migration. Scale bar = 200 μm; J and K: Tube formation assay to examine the formation of capillary-like structures by HUVECs. Scale bar = 200 μm. EdU: 5-Ethynyl-2’-deoxyuridine; NC: Negative control; CO 2 laser-Exos: Exosomes derived from CO 2 laser-preconditioned adipose-derived mesenchymal stem cells; AKT: Protein kinase B; p-AKT: Phosphorylated protein kinase B; HIF-1α: Hypoxia-inducible factor-1α; VEGF-A: Vascular endothelial growth factor-A.

Article Snippet: To evaluate the involvement of the PI3K/AKT signaling pathway in CO 2 laser-Exos-mediated angiogenesis, HUVECs were pretreated with either the AKT phosphorylation inhibitor LY294002 or the activator SC79 (MedChem Express, United States).

Techniques: Derivative Assay, Western Blot, Expressing, Negative Control, Cell Counting, Transwell Migration Assay, In Vitro, Wound Healing Assay, Migration, Tube Formation Assay